| Applications: |
Contains RGF BME qualified for low levels of tube formation in the absence of angiogenic factors and high levels of tube formation in the presence of angiogenesis factors, using human umbilical vascular endothelial cells (HUVEC)
Includes a tested inhibitor as a negative control
Contains both colorimetric and fluorometric cell staining reagents
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| |
| Applications: |
Detection of inducers and inhibitors of endothelial cell tube formation |
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| Components: |
Part # |
Component |
Size |
| |
3433-005-01* |
Cultrex BME; no phenol red; reduced growth factor |
5 ml |
| |
3470-096-01 |
Cell Staining Solution |
15 ml |
| |
3470-096-02 |
Sulforaphane 10mM |
15 µl |
| |
4892-010-01 |
Calcein AM |
50 µg |
| |
| Storage: |
Components are stored at -20ºC and at room temperature. |
| |
| References: |
1. Folkman J. 2007. Angiogenesis: an organizing principle for drug discovery? Nat. Rev. Drug Discov. 6:273-286.
2. Folkman J, Haudenschild C. 1980. Angiogenesis in vitro. Nature 288:551-556.
3. Kleinman HK, McGarvey ML, Liotta LA, Robey PG, Tryggvason K, Martin GR. 1982. Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21:6188-6193.
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