Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit Trevigen's Cultrex® In Vitro Angiogenesis Assays: Endothelial Cell Invasion kit was created in an effort to accelerate the screening process for compounds that influence vascular endothelial cell digestion through and migration across basement extracellular membrane (BME). This kit offers a flexible, standardized, high-throughput format for quantitating the degree to which endothelial cells penetrate a BME barrier, in vitro, in response to chemoattractants and/or inhibiting compounds. This assay employs a simplified Boyden chamber-like design with an 8 micron polyethylene terephthalate (PET) membrane. Sulforaphane [1-isothiocyanato-(4R)-methylsulfinyl)-butane], a naturally occurring cancer chemopreventive agent, is provided as a control for inhibition of in vitro endothelial cell migration/invasion on Cultrex® Basement Membrane Extract. Detection of cell invasion is quantified using Calcein-AM. Calcein AM is internalized by the cells, and intracellular esterases cleave the acetomethylester (AM) moiety. Free Calcein fluoresces brightly, and this fluorescence may be used to quantitate the number of cells that have invaded or migrated using a standard curve. Features: Includes a tested inhibitor as a negative control   Applications: Detection of inducers and inhibitors of endothelial cell invasion and migration   Components: Part # Component Size   3455-096-01 Cell Invasion Chamber each   3455-096-02 5X BME Solution 1 ml   3455-096-03 10X Coating Solution 1 ml   3455-096-04 25X Cell Wash Buffer 2 x 1.5 ml   3455-096-05 10X Cell Dissociation Solution 2 x 1.5 ml   3470-096-02 Sulforaphane 10mM 15 µl   4892-010-01 Calcein AM 50 µg   Storage: Components are stored at -20ºC and at room temperature.   References: 1. Hanahan D, Weinberg RA. (2000) The hallmarks of cancer. Cell 100:57-70. 2. Medina MA, Munoz-Chapuli R, Quesada AR. (2007) Challenges of antiantiogenic cancer therapy: trials and errors, and renewed hope. J Cell Mol Med 11:374-82. 3. Rhodes JM, Simons M. (2007) The extracellular matrix and blood vessel formation: not just a scaffold. J Cell Mol Med 11:176-205. Top   Ordering Information Catalog #: Price: 3471-096-K Cultrex® Endothelial Cell Invasion Kit, 96 Tests $340.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products Cultrex In Vitro Angiogenesis Assay - Tube Formation Kit Cultrex 96 Well Cell Migration Assay Cultrex 96 Well BME Cell Invasion Assay Cultrex 96 Well Laminin I Cell Invasion Assay Cultrex 96 Well Collagen I Cell Invasion Assay Cultrex 96 Well Collagen IV Cell Invasion Assay Cultrex 24 Well Cell Migration Assay CultreCoat® 24 Well BME-Coated Cell Invasion Assay Cultrex 24 Well BME Cell Invasion Assay Cultrex 24 Well Laminin I Cell Invasion Assay Cultrex 24 Well Collagen I Cell Invasion Assay Cultrex 24 Well Collagen IV Cell Invasion Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP