Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® TACS® XTT Cell Proliferation Assay The use of tetrazolium salts, including XTT (2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium-5-carboxanilide), to assay cell proliferation, cell viability, and/or cytotoxicity is a widespread, established practice. The procedures avoid radioactivity, allow for rapid determination in microplates, and give reproducible and sensitive results. Cleavage of the tetrazolium salt to formazan occurs via the succinatetetrazolium reductase system in the mitochondria of metabolically active cells. The reaction is attributed mainly to mitochondrial enzymes and electron carriers, but a number of other non-mitochondrial enzymes have been implicated. XTT, a yellow tetrazolium salt, is cleaved to a soluble orange formazan dye, which can be measured by absorbance at 490 (or 450) nm in a microplate reader. Efficient reduction of XTT requires an electron coupling reagent. This kit includes both XTT and the electron coupling reagent for a convenient and simple assay. Features: Sensitive. No radioactivity. Rapid (no solubilization step as in an MTT assay). Ideal for high throughput assays (no washing or other steps that can cause cell loss and variability).   Applications: Cell proliferation assays Cell viability assays Cytotoxicity analysis   Components: Part # Component Size   4891-025-01 XTT Reagent 5 x 25 ml   4891-025-02 XTT Activator 5 x 500 µl   Storage: Components are stored at -20°C.   Top   Ordering Information Catalog #: Price: 4891-025-K XTT Cell Proliferation Assay Kit, 2500 Tests $325.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products Cultrex Calcein-AM Cell Viability Assay MTT Cell Proliferation Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP