Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation CultreCoat® Collagen IV 96 Well Cell Adhesion Assay CultreCoat® Collagen IV Cell Adhesion Assay provides a simple, standardized, high throughput format for assessing factors that influence cell-matrix interactions. The black stripwell plate minimizes background, providing greater sensitivity, and affords flexibility for the number of samples assessed. The stripwell feature also allows multiple experiments to be conducted simultaneously using the same kit. Calcein labeling allows direct comparisons between the number of cells that are loaded and the number that adhere, providing a loading control and the percent cell adhesion based on the final fluorescence assessed for each well. Controls are provided for determining background and non-specific binding. Applications: Assess factors that influence cell-matrix interactions.   Components: Part # Component Size   3493-096-P Collagen IV 96 Well CA Plate 1 Plate   3490-096-01 2X CA Assay Buffer 100 ml   3490-096-02 10X CA Blocking Agent 3 ml   4892-010-01 Calcein AM 50 µg   Storage: Components are stored at -20ºC, 4ºC, and at room temperature.   References: 1. Dike, L.E. and D.E. Ingber, Integrin-dependent induction of early growth response genes in capillary endothelial cells. J Cell Sci, 1996. 109 (Pt 12): p. 2855-63. 2. Zhu, X., et al., Adhesion-dependent cell cycle progression linked to the expression of cyclin D1, activation of cyclin E-cdk2, and phosphorylation of the retinoblastoma protein. J Cell Biol, 1996. 133(2): p. 391-403. 3. Takeuchi, Y., et al., Differentiation and transforming growth factor-beta receptor down-regulation by collagen-alpha2beta1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells. J Biol Chem, 1997. 272(46): p. 29309-16. Top   Ordering Information Catalog #: Price: 3493-096-K CultreCoat® Collagen IV 96 Well Cell Adhesion Assay, 96 wells $195.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products CultreCoat® Laminin I 96 Well Cell Adhesion Assay CultreCoat® BME 96 Well Adhesion Assay CultreCoat® Vitronectin 96 Well Cell Adhesion Assay CultreCoat® Collagen I 96 Well Cell Adhesion Assay CultreCoat® Adhesion Protein Array Kit CultreCoat® Fibronectin 96 Well Cell Adhesion Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP