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Cultrex® Bovine Vitronectin NZHD*

Vitronectin is an extracellular soluble disulfide linked dimer composed of a 75 kDa and a 65 kDa peptide chain with a molecular weight of 140 kDa. Vitronectin is a major plasma glycoprotein that promotes cellular adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion or as an additive for serum-free medium.

Applications:
  • Cell adhesion
  • medium additive
  •  
    Preparation: Provided in 10 mM Sodium Phosphate, pH 7.7, 8M Urea, 5 mM EDTA, 500 mM NaCl at a concentration of 0.68 mg/ml.
     
    Performance Testing: Functionally tested for its ability to promote attachment of HT-1080 cells.
     
    Source: Bovine plasma from New Zealand**.
     
    Storage: Store at -20°C or -80°C in a manual defrost freezer. Avoid freeze-thaw cycles.
     
    References: Vuento M, Korkolainen M, Kuusela P, Holtta E. isolation of a novel cell-attachment and spreading-promoting protein from human serum, Biochem J. 1985 Apr 15; 227(2);421-7.

    Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E. Vitronectin - a major cell attachment-promoting protein in fetal bovine serum. Exp Cell Res. 1985 Oct; 160(2):245-58.

    **New Zealand was granted neglible BSE risk disease status at the 75th General Session of the World Organization for Animal Health (OIE) (http://www.oie.int/eng/info/en_statesb.htm), and GBR I status by the European Food Safety Authority (http://ec.europa.eu/food/fs/sc/ssc/out292_en.pdf). No cases of BSE have been reported in New Zealand since monitoring began in 1989 (http://www.oie.int/eng/info/en_esbcarte.htm).

    *NZHD = New Zealand Herd Derived
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    Ordering Information
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    Catalog #: Price:

    3417-001-01
    Cultrex Vitronectin,
    50 µg
    $140.00
    Add to Cart

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