Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® 96 Well Cell Migration Assay The Cultrex® Cell Migration Assay was created in an effort to accelerate the screening process for compounds that influence chemotaxis, which is a fundamental function of cellular processes such as angiogenesis, embryonic development, immune response, and wound healing. This assay offers a flexible, standardized, high throughput format for quantitating the degree to which cells migrate in vitro in response to chemoattractants and/or inhibiting compounds. The Cultrex® Cell Migration Assay utilizes a simplified boyden chamber design with an 8 micron polyethylene terephthalate (PET) membrane. Ports within the migration chamber (top) allow the access to the assay chamber (bottom) without dismantling the device. This design is easier to use, prevents contamination, and is adaptable for robotic high throughput systems. The assay chamber may be directly analyzed in a 96 well plate reader, eliminating transfer steps that introduce additional variability to the assay. Detection of cell migration is quantified using Calcein AM. Calcein AM is internalized by the cells, and intracellular esterases cleave the acetomethylester (AM). Free Calcein fluoresces brightly, and this fluorescence may be used to quantitate the number of cells that have migrated using a standard curve. Sufficient reagents are included to assess cell migration in 96 wells, as well as to calculate multiple standard curves. Applications: To quantitate cell migration   Components: Part # Component Size   3455-096-01 Cell Invasion Chamber each   3455-096-04 25X Cell Wash Buffer 2 x 1.5 ml   3455-096-05 10X Cell Dissociation Solution 2 x 1.5 ml   4892-010-01 Calcein AM 50 µg   Storage: Components are stored at -20ºC, 4ºC, and at room temperature.   References: 1. Tamilarasan KP, Kolluru GK, Rajaram M, Indhumathy M, Saranya R, Chatterjee S. Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cells. BMC Cell Biol. 2006 Apr 4;7:17. 2. Borghesani PR, Peyrin JM, Klein R, Rubin J, Carter AR, Schwartz PM, Luster A, Corfas G, Segal RA. BDNF stimulates migration of cerebellar granule cells. Development. 2002 Mar;129(6):1435-42. 3. Mohan K, Ding Z, Hanly J, Issekutz TB. IFN-gamma-inducible T cell alpha chemoattractant is a potent stimulator of normal human blood T lymphocyte transendothelial migration: differentialregulation by IFN-gamma and TNFalpha. J Immunol. 2002 Jun 15;168(12):6420-8. Trevigen Cell Assays Trevigen Cell Assays Top   Ordering Information Catalog #: Price: 3465-096-K Cultrex 96 Well Cell Migration Assay, 96 samples $295.00 Trevigen Products are now available for ordering through - AND - Download Protocol Technical Presentations   Related Products Cultrex 96 Well Endothelial Cell Invasion Kit Cultrex 96 Well BME Cell Invasion Assay Cultrex 96 Well Collagen I Cell Invasion Assay Cultrex 96 Well Collagen IV Cell Invasion Assay Cultrex 96 Well Laminin I Cell Invasion Assay Cultrex 24 Well BME Cell Invasion Assay Cultrex 24 Well Collagen I Cell Invasion Assay Cultrex 24 Well Collagen IV Cell Invasion Assay Cultrex 24 Well Laminin I Cell Invasion Assay CultreCoat® 24 Well BME-Coated Cell Invasion Assay Cultrex 24 Well Cell Migration Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP