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Cultrex® 24 Well Laminin I Cell Invasion Assay Kit

Trevigen’s Cultrex® Cell Invasion Assays were originally created in an effort to accelerate the screening process for compounds that influence cellular invasion through extracellular matrices, which is fundamental to angiogenesis, embryonic development, immune responses, and tumor cell metastasis4. To provide a platform for the analysis of responses to chemokines, toxins, drugs and other analytes of interest, for larger numbers of cells per well, Trevigen offers its Cultrex® 24 Well Laminin I Cell Invasion Assay kit. The modular format of the Cultrex® 24 Well Laminin I Cell Invasion Assay offers flexible utility, and sufficient insert size for informative results. The Cultrex® 24 Well Laminin I Cell Invasion Assay utilizes a simplified Boyden chamber design with an 8 µm polycarbonate (PC) membrane. Detection of cell invasion or migration is quantified using Calcein-AM. Calcein-AM is internalized by cells, and intracellular esterases cleave the acetomethylester (AM) moiety to generate free Calcein. Free Calcein fluoresces brightly, and this fluorescence may be used to quantitate the number of cells that have invaded or migrated using a standard curve.

Applications: Cell Migration/Invasion Assays
 
Components: Part # Component Size
  3455-024-01 24 well cell invasion/migration plates 2 Plates
  3455-096-03 10X Coating Solution 1 ml
  3455-096-04 25X Cell Wash Buffer 2 x 1.5 ml
  3455-096-05 10X Cell Dissociation Solution 2 x 1.5 ml
  3456-096-02 5X Laminin I Solution 1 ml
  4892-010-01 Calcein AM 50 µg
 
Storage: Components are stored at -20ºC, 4ºC, and at room temperature.
 
References: 1. Tamilarasan KP, Kolluru GK, Rajaram M, Indhumathy M, Saranya R, Chatterjee S. 2006. Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cells. BMC Cell Biol. 7:17.

2. Borghesani PR, Peyrin JM, Klein R, Rubin J, Carter AR, Schwartz PM, Luster A, Corfas G, Segal RA. 2002. BDNF stimulates migration of  cerebellar granule cells. Development 129:1435-1442.

3. Mohan K, Ding Z, Hanly J, Issekutz TB. 2002 IFN-gamma-inducible T cell alpha chemoattractant is a potent stimulator of normal human blood Tlymphocyte transendothelial migration: differential regulation by IFN-gamma and TNF-alpha. J Immunol. 168:6420-6428.

4. Li G, Chen YF, Greene GL, Oparil S, Thompson JA. 1999 Estrogen inhibits vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro. Circulation 100:1639-1645.
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Ordering Information
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Catalog #: Price:

3456-024-K
Cultrex™ 24 Well Laminin I Cell Invasion,
24 inserts, 2 plates
$370.00
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TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877
1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM