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Cultrex® 96 Well Laminin I Cell Invasion Assay

Cultrex® 96 Cell Invasion Assays were created in an effort to accelerate the screening process for compounds that influence cell migration through extracellular matrices. These assays offer a flexible, standardized, high throughput format for quantitating the degree to which invasive cells penetrate a barrier consisting of basement membrane components in vitro in response to chemoattractants and/or inhibiting compounds. The Cultrex® Cell Invasion Assay has been adapted to multiple formats so that cell invasion may be evaluated against different extracellular matrices; the assay is available in the following formats: Basement Membrane Extract (BME), Laminin I, Collagen I, Collagen IV.

Applications: Cell migration/Invasion Assays
 
Components: Part # Component Size
  3455-096-01 Cell Invasion Chamber each
  3455-096-03 10X Coating Solution 1 ml
  3455-096-04 25X Cell Wash Buffer 2 x 1.5 ml
  3455-096-05 10X Cell Dissociation Solution 2 x 1.5 ml
  3456-096-02 5X Laminin I Solution 1 ml
  4892-010-01 Calcein AM 50 µg
 
Storage: Components are stored at -20ºC, 4ºC, and room temperature.
 
References: Liotta, L.A. 1984. Tumor invasion and metastasis: role of the basement membrane. Am. J. Pathol. 117:339-348.

Terranova, V.P., E.S. Hujanen, D.M. Loeb, G.R. Martin, L. Thornburg, and V. Glushko. 1986. Use of reconstituted basement membrane to measure cell invasiveness and select for highly invasive tumor cells. Proc. Natl. Acad. Sci. USA83:465-469.

Albini, A., Y. Iwamoto, H.K. Kleinman, G.R. Martin, G.R. Aaronson, J.M. Kozlowski, and R.N. McEwan. 1987. A rapid in vitroassay for quantitating the invasive potential of tumor cells. Cancer Res. 47:3239-3245.
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Ordering Information
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Catalog #: Price:

3456-096-K
Cultrex 96 Well Laminin I Cell Invasion Assay,
96 samples
$340.00
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