| Applications: |
Coating tissue culture surfaces
Medium additive to promote cell adhesion and proliferation
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| Preparation: |
Provided in 0.05 M Phosphate, 0.725 M NaCl, pH=7. |
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| Purity: |
>90% by SDS-PAGE. Human fibronectin is tested negative by PCR, for seven different human pathogenic viruses including Hepatitis (A,B,C), HIV (1,2), Hantaan, Seoul, and Sin Nombre. |
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| Performance Testing: |
Functionally tested for its ability to promote attachment of Jurkat cells. |
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| Source: |
Human Plasma |
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| Storage: |
Store at 4°C. |
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| Safety Statement: |
Cultrex® Human Fibronectin, PathClear® is purified from human source material and therefore should be treated as potentially infectious and handled at Biological Safety Level 2 to minimize exposure. |
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| References: |
1. Vaheri A, Mosher DF. (1978) High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation. Biochim Biophys Acta. 516(1):1-25.
2. Mao Y, Schwarzbauer JE. (2005) Fibronectin fibrillogenesis, a cell-mediated matrix assembly process. Matrix Biol 24:389-99.
3. Polyak K, Weinberg RA. (2009) Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer 9:265-73.
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