| Applications: |
Coating tissue culture surfaces to promote cell adhesion, proliferation and differentiation
Additive for serum-free media.
|
| |
| Preparation: |
Provided in 10 mM Sodium Phosphate, pH 7.7, 8M Urea, 5 mM EDTA, 500 mM NaCl at a concentration of 1 mg/ml. |
| |
| Purity: |
>90% by SDS-PAGE. |
| |
| Performance Testing: |
Functionally tested for its ability to promote attachment of HT-1080 cells. |
| |
| Source: |
Human Plasma |
| |
| Storage: |
Store at -80°C in a manual defrost freezer. Avoid freeze-thaw cycles. |
| |
| Safety Statement: |
Cultrex® Human Vitronectin, PathClear® is purified from human source material and therefore should be treated as potentially infectious and handled at Biological Safety Level 2 to minimize exposure. |
| |
| References: |
1. Vuento M, Korkolainen M, Kuusela P, Holtta E. (1985) Isolation of a novel cell-attachment and spreading-promoting protein from human serum. Biochem J. 227:421-7.
2. Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E (1985). Vitronectin--a major cell attachment promoting protein in fetal bovine serum. Exp Cell Res. 160:245-58.
3. Tschopp J, Masson D, Schafer S, Peitsch M, Preissner KT (1988). The heparin binding domain of S protein/vitronectin binds to complement components C7, C8, and C9 and perforin from cytolytic T cells and inhibits their lytic activities. Biochemistry 27:4103-9.
|
