Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® TACS® MTT Cell Proliferation Assay The TACS® MTT Cell Proliferation Assay (MTT-CPA) is a sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt 3,[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). Changes in cell proliferative activity caused by trophic factors, growth inhibitors, or inducers and inhibitors of apoptosis, may be quantified using the MTT-CPA. MTT is reduced to an insoluble formazan dye by mitochondrial enzymes associated with metabolic activity. The reduction of MTT is primarily due to glycolytic activity within the cell and is dependent upon the presence of NADH and NADPH. Common methods for determining cell viability depend upon membrane integrity (e.g. trypan blue exclusion), or incorporation of nucleotides during cell proliferation (e.g. BrdU or 3H-thymidine). These methods are limited by the impracticality of processing large numbers of samples, or by the requirement for handling hazardous materials. The MTT Assay, in contrast, provides a rapid and versatile method for assessing cell viability. The assay is used to measure changes in cell proliferation. In actively proliferating cells, an increase in MTT conversion is spectrophotometrically quantified. Comparison of this value to an untreated control provides a relative increase in cellular proliferative activity. Conversely, in cells that are undergoing apoptosis, MTT reduction decreases, reflecting the loss of cell viability. Features: Convenient. Stabilized formulation is stored in your refrigerator and does not require thawing before use. Convenient. Stabilized formulation is stored in your refrigerator and does not require thawing before use. Fast. High throughput microplate format. Flexible. The reaction product can be visualized directly by microscopy to evaluate cell to cell reactivity, or solubilized and evaluated by microplate reading. Safe. Reaction product is solubilized using a non-organic solvent.   Applications: Cell proliferation assays Cytotoxicity analysis Apoptosis screening   Components: Part # Component Size   4890-025-01 MTT Reagent 25 ml   4890-025-02 Detergent Reagent 250 ml   Items Not Included: Microtiter plate   Storage: Components are stored at 4°C and room temperature.   Top   Ordering Information Catalog #: Price: 4890-25-K MTT Cell Proliferation Assay Kit, 2500 Tests $230.00 4890-50-K MTT Cell Proliferation Assay, 5000 Tests $350.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products Cultrex Calcein-AM Cell Viability Assay XTT Cell Proliferation Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP