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Mesenchymal Stem Cell Osteogenic Differentiation Kit

Mesenchymal Stem Cells can differentiate into osteogenic cells that will deposit and mineralize extracellular matrix proteins which are required for the formation of new bone. Measuring the amount of mineralized matrix is an established method for evaluating osteogenic differentiation. Trevigen's Mesenchymal Stem Cell Osteogenic Differentiation Kit contains reagents optimized to direct mesenchymal stem cells (MSC) grown on Cultrex® Rat Collagen I to undergo osteogenic differentiation in a defined growth medium supplemented with ascorbic acid, ß-glycerol phosphate, dexamethasone and Qualified fetal bovine serum within 14 days. Osteogenic differentiation is detected by staining with Alizarin Red S which stains the calcium deposits that occur when differentiated mesenchymal stem cells mineralize the extracellular matrix.

Applications: Tool for understanding mechanisms involved in bone formation and for identifying potential treatments for osteoporosis, bone repair and genetic disorders such as osteogenesis imperfecta.
 
Components: Part # Component1 Size
  5010-024-01 Dexamethasone* 12 µl
  5011-024-01 Ascorbic Acid 1.2 ml
  5011-024-02 ß-glycerol Phosphate 1.2 ml
  3440-001-01 Cultrex® Rat Collagen I2 500 µl
  5011-024-03 Alizarin Red S3 25 ml
  5011-024-04 Stain Solubilization Solution3 35 ml
  1: Reagents are sufficient to differentiate one 24 well plate
2: Sufficient rat tail collagen type I is provided to coat two 24 well plates
3: Enough reagent is provided to test two 24 well plates.
* Separate from shipping box and store at specified temperature
 
Storage: Components are stored at -20°C, 4°C, and at room temperature.
 
References: 1. Satija NK, Gurudutta GU, Sharma S, Afrin F, Gupta P, Verma K, Singh VK, Tripathi RP. 2007 Mesenchymal Stem Cells: Molecular Targets for Tissue Engineering. Stem Cells and Development 16:7-23.

2. Jones JL, Scotting P, Sottile V. 2007. Adult Mesenchymal stem cell: Differentiation potential and therapeutic applications. J Postgrad Med 53(2): 121-127.

3. Li F, Wang, X, Niyibizi C. 2007. Distribution of Single-Cell Expanded Marrow Derived Progenitors in a Developing Mouse Model of Osteogensis Imperfecta Following Systemic Treatment. Stem Cells. 25:3183-3193.
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Catalog #: Price:

5011-024-K
Mesenchymal Stem Cell Osteogenic Differentiation Kit,
24 wells
$395.00
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