spacer
spacer
 Trevigen
Shopping Cart Shopping Cart

spacer
spacer
spacer
Drop Down
Trevigen HomeCompanyProductsTechnical InformationDistributorSpacerLiterature
spacer
 » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA
 spacer
spacer
 spacer
 spacer
spacer
 spacer
 spacer
spacer
 Home » Apoptosis

Apoptosis

DASHTM (Diffusion Apoptosis Slide Halo) Assay Kit

Trevigen's new CometAssay Kit, DASH™ (Diffusion Apoptosis Slide Halo), allows discrimination of healthy and damaged cells in just a few hours. Simply embed cells in low melting agarose on a pre-treated slide, lyse under alkaline conditions, and precipitate the DNA of the remaining nuclear region (i.e. nucleoid) in the agarose. The precipitated DNA is visualized using **SYBR® Green I nucleic acid stain. When excited (425 - 500 nm) the DNA-bound **SYBR® Green I emits green light. In healthy cells, the fluorescence is confined to the nucleoid: undamaged DNA is supercoiled and thus does not diffuse very far, if at all, from the nucleoid. In cells that have accrued damage to the DNA, the alkali lysis treatment unwinds the DNA, releasing fragments that diffuse away from the center of the nucleoid, creating a "halo" surrounding a small compact origin. This kit includes ready to use, pre-coated slides and optimized reagents for your convenience.

Applications:
  • Detect and quantitate DNA damage
  • Identify apoptotic cells quickly and easily
  • Follow DNA repair
    •  
    Features:
  • Hydrophobic barrier allows easy application of cells directly to slide, and treatment with any of several Trevigen DNA repair enzymes.
  • Easy to use kit format.
  • Work with a small number of cells.
    •  
    Components: Part # Component Size
     
     
    Storage: Components are stored at -20°C or room temperature.
     
    References: Singh NP. (2000) A simple method for accurate estimation of apoptotic cells. Exp Cell Res. 256(1): 328-37

    Singh NP, Muller CH, Berger RE. (2003) Effects of age on DNA double-strand breaks and apoptosis in numan sperm. Fertil, Steril. 80(6): 1420-30.
    Top


    		 
    Ordering Information
    spacer
    Catalog #: Price:
    Trevigen Products are now available for ordering through
    DASH Assay
    Sample Data and Data Analysis of DASH Assay
    Click the image for more information
    Download Protocol
    data icon
    Related Products
    TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877
    1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM