| Features: |
Fast, sensitive detection of apoptotic cells.
Includes propidium iodide for discriminating apoptotic and necrotic (or late apoptotic) cells.
Annexin V-Biotin provides flexibility in fluorophore choice.
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| Applications: |
Flow cytometry
In situ detection of apoptotic cells
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Annexin V FITC Kits
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| Components: |
Part # |
Component |
Size |
| |
4830-01-01 |
Annexin V FITC (A) |
100 µl |
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4830-01-02 |
10X Binding Buffer (A) |
8 ml |
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4830-01-03 |
Propidium Iodide (A) |
1 ml |
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4830-250-01 |
Annexin V FITC (B) |
250 µl |
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4830-250-02 |
10X Binding Buffer (B) |
20 ml |
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4830-250-03 |
Propidium Iodide (B) |
2.5 ml |
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(A) 4830-01-K Annexin V-FITC 100 tests
(B) 4830-250-K Annexin V-FITC Kit 250 tests
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Annexin V Biotin Kits
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| Components: |
Part # |
Component |
Size |
| |
4830-01-02 |
10X Binding Buffer (A) |
8 ml |
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4830-01-03 |
Propidium Iodide (A) |
1 ml |
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4830-250-02 |
10X Binding Buffer (B) |
20 ml |
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4830-250-03 |
Propidium Iodide (B) |
2.5 ml |
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4835-01-01 |
Annexin V Biotin (A) |
100 µl |
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4835-250-1 |
Annexin V Biotin (B) |
250 µl |
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(A) 4835-01-K Annexin V-Biotin 100 tests
(B) 4835-250-K Annexin V-Biotin 250 tests
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| Storage: |
Store at 4°C. |
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| References: |
1. Fadock, V.A., D.R. Voelker, P.A .Campbell, J.J. Cohen, D.L. Bratton, and P.M. Henson. 1992. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 148:2207-2216.
2. Tait, J.F., and D. Gibson. 1992. Phospholipid binding of Annexin V: Effects of calcium and membrane phosphatidylserine content. Arch Biochemiand Biophys 298(1):187-191.
3. Meers, P., and T. Mealy. 1993. Calcium-dependent Annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge. Biochemistry 32:11711-11721.
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