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Apoptosis

TACS® Annexin V Kits

TACS® Annexin V Kits allow rapid, specific, and quantitative identification of apoptosis in individual cells. Annexin V is a calcium-dependent phospholipid binding protein. During apoptosis, an early and ubiquitous event is the exposure of phosphatidylserine at the cell surface. Trevigen offers annexin V conjugated to either FITC or to biotin for the detection of cell surface changes during apoptosis. The annexin V conjugates are supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, an indication of late apoptosis or necrosis.

Features:
  • Fast, sensitive detection of apoptotic cells.
  • Includes propidium iodide for discriminating apoptotic and necrotic (or late apoptotic) cells.
  • Annexin V-Biotin provides flexibility in fluorophore choice.
  •  
    Applications:
  • Flow cytometry
  • In situ detection of apoptotic cells
  •  
      Annexin V FITC Kits
    Components: Part # Component Size
      4830-01-01 Annexin V FITC (A) 100 µl
      4830-01-02 10X Binding Buffer (A) 8 ml
      4830-01-03 Propidium Iodide (A) 1 ml
      4830-250-01 Annexin V FITC (B) 250 µl
      4830-250-02 10X Binding Buffer (B) 20 ml
      4830-250-03 Propidium Iodide (B) 2.5 ml
     
      (A) 4830-01-K Annexin V-FITC 100 tests
    (B) 4830-250-K Annexin V-FITC Kit 250 tests
     
      Annexin V Biotin Kits
    Components: Part # Component Size
      4830-01-02 10X Binding Buffer (A) 8 ml
      4830-01-03 Propidium Iodide (A) 1 ml
      4830-250-02 10X Binding Buffer (B) 20 ml
      4830-250-03 Propidium Iodide (B) 2.5 ml
      4835-01-01 Annexin V Biotin (A) 100 µl
      4835-250-1 Annexin V Biotin (B) 250 µl
     
      (A) 4835-01-K Annexin V-Biotin 100 tests
    (B) 4835-250-K Annexin V-Biotin 250 tests
     
    Storage: Store at 4°C.
     
    References: 1. Fadock, V.A., D.R. Voelker, P.A .Campbell, J.J. Cohen, D.L. Bratton, and P.M. Henson. 1992. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 148:2207-2216.
    2. Tait, J.F., and D. Gibson. 1992. Phospholipid binding of Annexin V: Effects of calcium and membrane phosphatidylserine content. Arch Biochemiand Biophys 298(1):187-191.
    3. Meers, P., and T. Mealy. 1993. Calcium-dependent Annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge. Biochemistry 32:11711-11721.
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    Ordering Information
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    Catalog #: Price:

    4830-01-K
    Annexin V-FITC 100 tests,
    100 Samples
    $305.00
    Add to Cart

    4830-250-K
    Annexin V-FITC 250 tests,
    250 Samples
    $655.00
    Add to Cart

    4835-01-K
    Annexin V-Biotin 100 tests,
    100 Samples
    $340.00
    Add to Cart

    4835-250-K
    Annexin V-Biotin 250 tests,
    250 Samples
    $675.00
    Add to Cart

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