Trevigen, Inc. | Apoptosis


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Apoptosis

CometAssay^TM Kit

Trevigen’s CometAssay^™ provides reagents and our exclusive CometSlide^™ for the rapid analysis of DNA fragmentation associated with DNA damage. The comet assay or single cell gel electrophoresis assay is based on the alkaline lysis of labile DNA at sites of damage. The unwound, relaxed DNA is able to migrate out of the cell during electrophoresis and can be visualized using **SYBR^® Green I nucleic acid gel stain. Cells that have accumulated DNA damage appear as fluorescent comets with tails of DNA fragmentation or unwinding, whereas, normal undamaged DNA does not migrate far from the origin. The CometAssay is provided with our exclusive CometSlides which greatly simplify the assay. Each slide provides two sample surfaces specially treated to promote agarose adherence, and a hydrophobic barrier to allow treatment with one of Trevigen’s DNA repair enzymes. Simply add your cells to the low melting point Comet LMAgarose, and pipet onto the slide.

Features:	CometSlides allow direct application of Comet LMAgarose without base layers or pretreatment. Hydrophobic barrier allows easy application of cells directly to slide, and treatment with any of several Trevigen DNA repair enzymes. Easy to use kit format.

Applications:	Detect and quantitate DNA damage Identify apoptotic cells quickly and easily Follow DNA repair Higher Throughput CometAssay

Components:	Part #	Component	Size

Storage:	Components are stored at -20�C or room temperature.

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TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877
1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM