Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » Apoptosis Apoptosis DermaTACSTM In Situ Apoptosis Detection Kits The DermaTACS™ Kit was developed to provide researchers with an effective method for measuring apoptosis in skin samples. The kit was based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and modified nucleotides. Detection of incorporated molecules is achieved using a chromogenic substrate with a horseradish peroxidase detection system. The extracellular scaffolding in skin tissue can make it problematic to permeabilize and high background is a common problem with skin samples. The DermaTACS Kit includes two permeabilization reagents as options for permeabilization along with a detailed protocol with hints and tips for optimal labeling of skin samples based on empirical testing. Low background is achieved using a combination of the high specificity of Trevigen’s unique cation based optimized TdT labeling reaction using Apoptosis Grade™ TdT and TACS Blue Label™ as the visualization method. Also included is TACS-Nuclease™ to generate a positive control from your own sample. In conjunction with the excellent contrast between the TACS Blue Label and the Red Counterstain C, the positive control ensures easy data interpretation. Features: Fast. Requires less than 3 hours to complete. Exclusive, non-toxic TACS Safe TdTTM buffer - sodium cacodylate free. Unique buffer system produces more consistent labeling. Performance tested on skin derived samples. Exclusive CytoninTM permeabilization reagent included. TACS-Nuclease solution for preparing sample-dependent positive controls included.   Applications: TUNEL Assay In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Assists in the identification of apoptotic morphologies. Helps resolve unique problems encountered when detecting apoptosis in skin sections.   Components: Part # Component Size   4800-30-01 Proteinase K 30 µl   4800-30-06 Strep-HRP 30 µl   4800-30-11 TACS Blue Label™ 3 ml   4800-30-15 TACS-Nuclease™ 15 µl   4800-30-16 TACS-Nuclease Buffer 15 µl   4800-30-19 Red Counterstain C 50 ml   4810-30-02 TACS 2 TdT Labeling Buffer 100 ml   4810-30-03 TACS 2 TdT Stop Buffer 100 ml   4810-30-05 TdT Enzyme 30 µl   4828-30-04 TACS B-dNTP Mix 30 µl   4828-30-06 Anti-BrdU antibody 30 µl   4828-30-12 Strep-Diluent 7.5 ml   4876-05-01 Cytonin™ 5 ml       Storage: Components are stored at -20°C, 4°C, and room temperature.   References: 1. Dakhama, A. R.G. Hegele (1996) A nonradioactive method for rapid and sensitive detection of polymerase chain reaction products by use of bromodeoxyuridine. Modern Pathology 9: 849-853 2. Gavrieli, Y., Y. Sherman, S.A. Ben-Sasson (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol.119:493- 501 3. Gratzner, H.G. (1982) Monoclonal antibody to 5-bromo and 5-iodoodeoxyuridine. A new reagent for etection of DNA replication. Science 218:474. 4. Kerr, J.F., G.C. Gobe, C.M. Winterford and B.V. Harmon (1995) Anatomical methods in cell death. Methods in Cell Biology 46:1-27. Top   Ordering Information Catalog #: Price: 4800-30-42 EpiDerm Control Slides, 2 each $40.00 4829-30-K DermaTACS Kit, 30 Samples $350.00 Trevigen Products are now available for ordering through Download Protocol Select Protocol 4829-30-K 4800-30-42 Related Products CardioTACS Kit VasoTACS Kit HT TiterTACS Kit NeuroTACS II Kit FlowTACS Kit TumorTACS Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM