FlowTACS® Apoptosis Detection Kits
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FlowTACS® takes advantage of Trevigen’s exclusive in situ labeling technology to label cell samples for processing
by flow cytometry. FlowTACS® also provides flexibility in selection
of fluorophores that are compatible with your research design.
The kit allows multi-color labeling in conjunction with experiment
specific antibodies.
DNA fragmentation is a committed step in apoptosis, and the
labeling of 3’ ends provides an easy measure of cells
undergoing apoptosis. Cells may also be analyzed for DNA content
using the included propidium iodide/RNase A solution. The
FlowTACS® Kit also provides TACS-Nuclease™ to generate
positive controls for calibrating your flow cytometer. The
FlowTACS® Kit uses fixed cells, allowing you to safely work
with cells that are infected with biohazardous agents. Also,
samples may be stored conveniently during time-course experiments.
This complete kit provides all the reagents required for
labeling including two permeabilization reagents, labeling
and stop buffers, labeling and detection reagents, and TACS-Nuclease
for generating positive controls with your own samples. Please
refer to our components listing for details.
| Features: |
Fast. Requires less than 3 hours to complete.
Exclusive, non-toxic TACS® Safe TdTTM buffer - sodium cacodylate free.
Unique buffer system produces more consistent labeling.
Works on fixed cells.
Includes exclusive CytoninTM permeabilization reagent.
Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
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| Applications: |
Identify and quantitate apoptotic cells in culture.
Bi-color and tri-color analysis.
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| Components: |
Permeabilization reagents
Optimized cation
TACS-Nuclease
Labeling buffer
Detection reagents
Propidium iodide/RNase solution
Nucleotide mix
Fluorescent label
Labeling enzyme
Counterstain
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| Items Not Included: |
Reagents for dehydration and rehydration,
H2O2, PBS buffer, and disposables.
Centrifuge equipped with microplate adapters.
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| Storage: |
Components are stored at -20°C, 4°C, and room temperature. |
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