| Features: |
Fast. Requires less than 3 hours to complete.
Exclusive, non-toxic TACS Safe TdTTM buffer - sodium cacodylate free.
Unique buffer system produces more consistent labeling.
Exclusive NeuroPore permeabilization reagent.
TACS-Nuclease solution for preparing sample-dependent positive controls.
DAB Enhancer for intensifying and darkening DAB staining.
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| Applications: |
TUNEL Assay
In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Assists in the identification of apoptotic morphologies.
Helps resolve unique problems encountered when detecting apoptotic neuronal cells.
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| Components: |
Part # |
Component |
Size |
| |
4800-30-01 |
Proteinase K |
30 µl |
| |
4800-30-06 |
Strep-HRP |
30 µl |
| |
4800-30-07 |
Diaminobenzidine & Enhancer |
3.75 ml |
| |
4800-30-15 |
TACS-Nuclease™ |
15 µl |
| |
4800-30-16 |
TACS-Nuclease Buffer |
15 µl |
| |
4810-30-02 |
TACS 2 TdT Labeling Buffer |
100 ml |
| |
4810-30-03 |
TACS 2 TdT Stop Buffer |
100 ml |
| |
4810-30-04 |
TACS 2 TdT dNTP |
30 µl |
| |
4810-30-05 |
TdT Enzyme |
30 µl |
| |
4810-30-14 |
Manganese Cation (Sold as 4810-90-14) |
30 µl |
| |
4820-30-01 |
NeuroPore™ |
5 ml |
| |
4820-30-13 |
Blue Counterstain |
50 ml |
| |
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| Storage: |
Components are stored at -20°C, 4°C, and room temperature.
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| References: |
1. Brunstrom, J.E., M.R. Gray-Swain, P.A. Osborne, and A.L. Peariman (1997) Neuronal Heterotopias in the Developing Cerebral Cortex produced by Neurotrophin-4. Neuron 18:505-517.
2. Dakhama, A. R.G. Hegele (1996) A nonradioactive method for rapid and sensitive detection of polymerase chain reaction products by use of bromodeoxyuridine. Modern Pathology 9: 849-853
3. Gavrieli, Y., Y. Sherman, S.A. Ben-Sasson (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol.119:493-501
4. Gratzner, H.G. (1982) Monoclonal antibody to 5-bromo and 5- iodoodeoxyuridine. A new reagent for etection of DNA replication. Science 218:474.
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immunohistochemistry
and to reduce background staining. DNA fragments generated
by apoptosis are end-labeled with modified nucleotides using
a highly purified terminal deoxynucleotidyl transferase enzyme
(TdT). The incorporated nucleotides are detected using a horseradish
peroxidase system that catalyzes the conversion of diaminobenzidine
(DAB) into a dark brown precipitate. NeuroTACS II contains
the Blue Counterstain to allow visualization of all cells
within the sample with good contrast to the brown DAB precipitate.
In addition, the Blue Counterstain is compatible with the
red colored substrates used for phosphatase detection in double
labeling experiments. The protocol includes details for labeling
in situ for apoptosis and antigen detection on the same sample.
To ensure that your own samples have been processed and permeabilized
correctly, we provide TACS-Nuclease™, a unique reagent
used to generate a positive control with your own samples.
This provides a high degree of confidence for data interpretation
and can help pinpoint problem steps in the labeling procedure.
