The water is purified through a reverse osmosis system and multistage nanoparticle filtration system assuring greater than 18 megohm-cm² resistivity is provided in a convenient 500 ml size. The water is then sterilized using a unique autoclaving process to assure the highest quality.
 

Trevigen offers several different types of control slides to develop familiarity with the methods of in situ apoptosis labeling and to determine if reagents are working optimally. The Cell Culture Control Slides are useful for researchers studying apoptosis in cell culture sys-tems, whereas the Tissue Control Slides are helpful when investigating tissue specimens. EpiDerm^™ Control slides (Cat# 4800-30-42) are sections of synthetic human skin tailored for use with DermaTACS^™.

TACS Nuclease and Buffer

TACS-Nuclease is a novel DNA endonuclease that is used to prepare a positive control from your own samples. The nuclease can simply be added to the labeling reaction mixture or if preferred in a separate step. DNA fragments formed by the action of this enzyme serve as substrates for labeling, producing a positive signal in virtually every cell.
 

Trevigen offers a variety of counterstains for use in our TUNEL assay kits. Blue
counterstain unlike Methyl Green works well in neuronal tissue, and is also a good choice for double-labeling with brown or red detection systems. Nuclear Fast Red is the ideal counterstain for Trevigen kits employing the TACS^™ Blue Label chromogenic substrate. Red Counterstain C provides an alternative for a red counterstain. Our Methyl Green stain is free of cresyl violet contamination and provides excellent contrast with DAB detection systems.
 

Trevigen Mounting Medium is a high quality, optically clear, toluene based mounting medium. This mounting medium is ideal for mounting samples processed with our TACS^™ In Situ Apoptosis Detection kits using either diaminobenzidine or TACS Blue Label^™. Mounting samples helps protect the specimen from damage and helps produce a clear image for microscopic visualization and photography. For fluorescent samples, use Trevigen Fluorescence Mounting Medium (Cat# 4866-20).

Trevigen Fluorescence Mounting Medium

Trevigen Fluorescence Mounting Medium is specially designed for use with our In Situ Apoptosis Detection kits that use fluorescein as the detection method. The mounting medium hardens completely in a short time, preserving the sample and providing an air-tight seal under the glass coverslips.
 

Proteinase K is used for permeabilizing cells and tissues prior to labeling using any of the TACS^™ In Situ Apoptosis Detection Kits.

NeuroPore^™

NeuroPore is a non-proteolytic permeabilization and blocking reagent developed for use with the NeuroTACS^™ In Situ Apoptosis Detection kit (Cat# 4820-30-K). Many tissues of the central nervous system are fragile and do not tolerate harsh proteolytic treatment. NeuroPore provides a gentle method for the permeabilization of tissues prior to in situ labeling. In addition, NeuroPore is an ideal antibody diluent for use in double labeling experiments: antigenic determinants are retained during permeabilization and can be detected using standard immunohistochemical techniques in conjunction with in situ detection of apoptosis.

Cytonin^™

Cytonin offers an alternative to Proteinase K for permeabilizing cells and tissues prior to labeling using any of the TACS^™ In Situ Apoptosis Detection kits. Cytonin is a protease free, saponin-based buffer designed specifically for in situ detection of apoptosis. Cytonin should be used when protease treatment must be avoided. For example, it may be used for tissues with low cellularity or little connective tissue that do not withstand protease treatment. Cytonin IHC (see below) is recommended for double labeling experiments for the detection of DNA fragmentation in conjunction with immunohistochemistry on antigens that are sensitive to proteolysis.

Cytonin IHC^™

Cytonin-IHC a non-proteolytic, saponin-based permeabilization and blocking reagent designed specifically for double labeling experiments using TACS In Situ Apoptosis Detection kits, and a primary antibody of your choice. Cytonin IHC is used as a convenient time-saving antibody diluent for immunohistochemistry, allowing you to simultaneously perform your primary antibody incubation and permeabilization of your sample for in situ detection of apoptosis. In fact, use Cytonin IHC as a diluent for your secondary antibody too.
 

These slides are specially treated to promote electrostatic adhesion of your tissue or cell samples. The slides are frosted on one end allowing you to clearly identify each sample by labeling with a pencil or histology pen. The slides are precleaned and ready for use.

Treated Glass Microscope Slides with 3 Ring Hydrophobic Barrier

These slides have the same coating as our treated glass microscope slides (Cat# 4861-100) with an added 3 ring hydrophobic barrier allowing you to process 3 different samples easily on one slide. The barrier promotes better sample coverage and conserves reagents. Also, the barrier makes the task of drying suspension cells onto slides as easy as pipetting.

Hydrophobic Coverslips 22 x 40 mm

These coverslips are made of a specially treated rigid plastic that produces a hydrophobic surface that assures rapid and even distribution of reagents over the entire sample being labeled. The coverslips should be used to conserve reagents when larger sample areas are being labeled. Unlike glass coverslips that may not wet evenly and may be too heavy to allow sufficient reagent to contact the sample, these coverslips float easily on small volumes of labeling reagents. Unlike wax film or other commercially available coverslips, these coverslips are rigid, preventing uneven distribution of labeling solutions. The coverslips are packaged in rows of 5 with a protective film coating.

Glass Coverslips 22 x 60 mm

These coverslips are optically clear glass ideal for mounting onto your specimens prior to viewing. The coverslips are clean and ready for use. The 22 x 60 mm size assures complete coverage when using our 3 ring barrier slides (Cat# 4864-100), or when large samples are being processed. These may be used with Trevigen Mounting Medium (Cat# 4865-25), or Trevigen Fluorescence Mounting Medium (Cat# 4866-20).
 

Trevigen offers a number of streptavidin conjugates to allow flexibility in both in situ labeling, and our flow cytometric based assays. Optimal concentrations range from 1:50 dilutions to 1:1000 dilutions, depending upon application. The table below summarizes the characteristics of each conjugate.