TACS® 2 TdT In Situ Apoptosis Detection Kits
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The TACS® 2 TdT Kits utilize Trevigen’s unique cation
optimization system to enhance labeling within particular
tissues. The TACS® 2 TdT Kits employ a proprietary labeling
buffer that contains no toxic components (e.g. sodium cacodylate).
A highly purified form of the TdT enzyme is included in the
kits for the enzymatic incorporation of biotinylated nucleotides.
Biotin labeling is achieved using streptavidin-horseradish
peroxidase, and colorimetric substrates  diaminobenzidine
(DAB) or TACS® Blue Label™. For fluorescent detection,
a fluorescein conjugate of streptavidin is used and visualized
by epifluorescence microscopy. Additional fluorophores are
now available separately from Trevigen.
These complete kits provide all the reagents required for
labeling including two permeabilization reagents, labeling
and stop buffers, labeling and detection reagents, and TACS-Nuclease™
reagents for generating positive controls with your own samples.
Please refer to our components listing for details.
The core kit is for researchers familiar with in situ labeling
who are already set up for immunohistochemistry. A replenisher
kit is also available for users of our TACS® kits who have
used up the limiting reagents within the kit. This kit provides
a cost effective replacement of the reagents that are used
most quickly.
| Features: |
TdT based in situ labeling.
Cation optimization for high signal and low background.
Complete kit provides either DAB, TACS® Blue Label, or fluorescence detection options.
Includes exclusive CytoninTM permeabilization reagent.
Includes TACS-Nuclease control reagents.
DAB kit now includes a DAB enhancer to intensify and darken DAB staining!
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| Applications: |
In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Light microscopy
Fluorescence microscopy
Flow cytometry
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| Items Not Included: |
Reagents for dehydration and rehydration, H2O2, PBS buffer, and disposables
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| Storage: |
Components are stored at -20°C, 4°C, and room temperature. |
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