TACS•XL® In Situ Apoptosis Detection Kits
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TACS•XL® embodies a new approach for the in situ detection
of apoptosis. The TACS•XL kit is based on incorporation
of bromodeoxyuridine (BrdU) at the 3’ OH ends of the
DNA fragments that are formed during apoptosis. The incorporation
of BrdUTP by TdT is more efficient than either biotinylated
or digoxigenin labeled nucleotides used in other TUNEL-based
assays. The detection system utilizes a biotin conjugated
anti-BrdU antibody and streptavidin-horseradish peroxidase.
The combination of antibody specificity with the signal enhancing
properties of biotin:streptavidin results in precise cellular
labeling and the highest signal to noise ratio observed in
competitive testing.
Three kit formats are available: TACS•XL DAB, TACS•XL Blue Label and TACS•XL Basic. The TACS•XL DAB kit
is supplied with DAB and Methyl Green counterstain, and the
TACS•XL Blue Label kit is provided with TACS® Blue Label
and Nuclear Fast Red counterstain. These complete kits provide
all the reagents required for labeling including two permeabilization
reagents, labeling and stop buffers, labeling and detection
reagents, and TACS-Nuclease™ reagents for generating
positive controls with your own samples. The TACS•XL Basic kit provides the labeling reagents necessary for routine
labeling, and is ideal for researchers whose labs are equipped
for standard immunohistochemical procedures involving horseradish-peroxidase.
Please refer to our components listing for details.
| Features: |
High signal to noise ratio generates stronger signal with less background.
Less sensitive to protease-induced false positive labeling than digoxigenin or biotin-based kits.
Complete kit provides either DAB or TACS® Blue Label detection options.
Includes exclusive Cytonin™ permeabilization reagent.
Includes TACS-Nuclease control reagents.
Readily adapted for fluorescence read-out.
DAB kit now includes a DAB enhancer to intensify and darken DAB staining!
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| Applications: |
In situ detection of apoptosis in fixed frozen or paraffin embedded samples.
Assists in the identification of apoptotic morphologies.
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| Items Not Included: |
Reagents for dehydration and rehydration, H2O2, PBS buffer, and disposables |
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| Storage: |
Components are stored at -20°C, 4°C, and room temperature. |
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