HT TiterTACS® Colorimetric Apoptosis Detection Kits
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The HT TiterTACS® Colorimetric Apoptosis Detection Kit takes
advantage of Trevigen’s exclusive in situ labeling technology
bringing it to the 96 well microplate format for high throughput
quantitative detection of apoptosis. Detection using TACS-Sapphire™,
a non-toxic colorimetric substrate, allows both kinetic and
endpoint readings. The reaction generates a blue product that
can be measured at 630 nm, and will turn yellow after the
reaction is stopped with acid allowing endpoint reading at
450 nm.
During the process of apoptosis, DNA fragmentation occurs
following the activation of endonucleases. The labeling of
the 3’ ends of DNA fragments provides an easy measure
of cells undergoing apoptosis. Modified nucleotides are incorporated
at the 3’ ends by the activity of terminal deoxynucleotidyl
transferase (TdT). These nucleotides are detected using a
horseradish-peroxidase detection system and TACS-Sapphire™.
HT TiterTACS® can be used with suspension or monolayer cells.
The kit is designed to use fixed samples, allowing you to
work safely with samples that are infected with biohazardous
agents. Fixed samples may be stored conveniently during time-course
experiments.
The HT TiterTACS® Kit also provides TACS-Nuclease™ solution
to generate positive controls from your own samples giving
you a maximal value for the assay.
| Features: |
Fast. Requires less than 4 hours to complete.
Exclusive, non-toxic TACS® Safe TdT buffer - sodium cacodylate free.
Unique buffer system produces more consistent labeling.
Includes exclusive CytoninTM permeabilization reagent.
Includes TACS-NucleaseTM solution for preparing sample-dependent positive controls.
Convenient, 96 well microplate format.
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| Applications: |
Identification and quantitation of apoptosis in cultured cells.
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| Components: |
Permeabilization reagents
Labeling enzyme
Chromogenic substrate
Labeling buffer
Optimized cation
TACS-Nuclease
Nucleotide mix
Detection reagents
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| Items Not Included: |
Reagents for dehydration and rehydration,
H2O2, PBS buffer, and disposables. Centrifuge equipped with
microplate adapters.
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| Storage: |
Components are stored at -20°C, 4°C, and room temperature. |
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