Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Apoptosis Apoptosis VasoTACS® In Situ Apoptosis Detection Kits The VasoTACS® Kit was developed to provide an effective method for identifying apoptotic cells in vascular samples. This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS® Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS® Blue Label™ detection system. Trevigen’s exclusive apoptosis grade TdT enzyme coupled with the TACS® Blue Label solution provides a highly sensitive labeling method. Specifically, VasoTACS® has been tested and optimized in order to help eliminate background and improve labeling in vascular tissue. Included in this kit is Red Counterstain C, an excellent contrast to the TACS® Blue Label, as well as TACS-Nuclease™ which is used to generate a positive control in your own sample. The positive control provides an internal control for permeabilization and labeling so optimization is nominal and the data can be interpreted with confidence. Features: Fast. Requires less than 3 hours to complete. Exclusive, non-toxic TACS® Safe TdTTM buffer - sodium cacodylate free. Unique buffer system produces more consistent labeling. Performance tested on vascular tissues. Exclusive CytoninTM permeabilization reagent included.   Applications: TUNEL Assay In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded vascular cells and tissues Assists in the identification of apoptotic morphologies Helps resolve unique problems encountered when detecting apoptotic vascular cells.   Components: Part # Component Size   4800-30-01 Proteinase K 30 µl   4800-30-06 Strep-HRP 30 µl   4800-30-11 TACS Blue Label™ 3 ml   4800-30-12 Blue Strep-HRP Diluent 7.5 ml   4800-30-15 TACS-Nuclease™ 15 µl   4800-30-16 TACS-Nuclease Buffer 15 µl   4800-30-19 Red Counterstain C 50 ml   4810-30-02 TACS 2 TdT Labeling Buffer 100 ml   4810-30-03 TACS 2 TdT Stop Buffer 100 ml   4810-30-04 TACS 2 TdT dNTP 30 µl   4810-30-05 TdT Enzyme 30 µl   4810-30-14 Manganese Cation (Sold as 4810-90-14) 30 µl   4876-05-01 Cytonin™ 5 ml     Storage: Store components at -20°C, 4°C, and room temperature.   References: 1. Brunstrom, J.E., M.R. Gray-Swain, P.A. Osborne and A.L. Peariman (1997) Neuronal Heterotopias in the Developing Cerebral Cortex produced by Neurotrophin-4. Neuron 18:505-517. 2. Gavrieli, Y., Y. Sherman, S.A. Ben-Sasson (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol.119:493- 501 3. Kerr, J.F., G.C. Gobe, C.M. Winterford and B.V. Harmon (1995) Anatomical methods in cell death. Methods in Cell Biology 46:1-27. 4. Migheli, A., A. Attanasio, W.-H. Lee, S.A. Bayer and B. Ghetti (1995) Detection of apoptosis in weaver cerebellum by electron microscopic in situ end-labeling of fragmented DNA. Neurosci. Lett. 199:53-56. Top   Ordering Information Catalog #: Price: 4826-30-K VasoTACS Kit, 30 Samples $350.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products CardioTACS Kit FlowTACS Kit TumorTACS Kit DermaTACS Kit HT TiterTACS Kit NeuroTACS II Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP