| Features: |
Chemiluminescent, non-radioactive format
High throughput 96 test size
Sensitivity down to 0.1 mUnits of PARP--equivalent to less than 500 cells/well
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| Applications: |
Measuring PARP activity in cell extracts before and during apoptosis
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| Components: |
Part # |
Component |
Size |
| |
4671-096-02 |
*20X PARP Buffer |
500 µl |
| |
4671-096-06 |
*10X Activated DNA |
300 µL |
| |
4675-096-01 |
PeroxyGlowTM A |
6 ml |
| |
4675-096-02 |
PeroxyGlowTM B |
6 ml |
| |
4678-096-P |
*Histone Coated Strip Wells (white) |
96 wells |
| |
4684-096-01 |
PARP HSA, 10 units/µl |
100 µl |
| |
4684-096-02 |
20 mM NAD |
300 µl |
| |
4684-096-03 |
5X Antibody Diluent |
3 ml |
| |
4684-096-04 |
Anti-PAR Monoclonal Antibody |
20 µl |
| |
4684-096-05 |
HRP Conjugate Diluent |
20 µl |
| |
4684-096-06 |
10 mM Etoposide |
100 µl |
| |
*Components marked with an asterisk can be stored at -20°C for one year. |
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| Storage: |
Components are stored at -80°C and 4°C. |
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| References: |
1. Lawen A. (2003) Apoptosis—an introduction. BioEssays 25:888-896.
2. Okada H, Mak TW. (2004) Pathways of apoptotic and non-apoptotic death in tumor cells. Nat. Rev. Cancer 4:592-603.
3. Yu S-W, Wang H, Poitras MF, Coombs C, Bowers WJ, Federoff HJ, Poirier GG, Dawson TM, Dawson VL. (2002) Mediation of poly(ADP-ribose) polymerase-1- dependent cell death by apoptosis-inducing factor. Science 297:259-263.
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