Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage HT Colorimetric PARP/Apoptosis Assay During apoptosis, PARP-1 which catalyzes the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from about 116 kDa to 85 kDa. Trevigen's HT PARP/Apoptosis Assay is ideal for measuring the activity of PARP in cell extracts before and during apoptosis. The HT PARP/Apoptosis Assay is an ELISA which semi-quantitatively detects PAR deposited onto immobilized histone proteins in a 96-well format. An anti-PAR monoclonal antibody, goat anti-mouse IgG-HRP conjugate, and HRP substrate are used to generate a colorimetric signal. Thus, absorbance correlates with PARP activity. Etoposide is a topoisomerase II inhibitor that stabilizes this enzyme after it cleaves DNA. It is included as a control apoptosis inducer. Features: Colorimetric, non-radioactive format High throughput 96 test size Sensitivity down to 0.1 mUnits of PARP--equivalent to less than 500 cells/well   Applications: Measuring PARP activity in cell extracts before and during apoptosis   Components: Part # Component Size   4671-096-02 *20X PARP Buffer 500 µl   4671-096-06 *10X Activated DNA 300 µL   4678-096-P *Histone Coated Strip Wells (white) 96 wells   4684-096-01 PARP HSA, 10 units/µl 100 µl   4684-096-02 20 mM NAD 300 µl   4684-096-03 5X Antibody Diluent 3 ml   4684-096-04 Anti-PAR Monoclonal Antibody 20 µl   4684-096-05 HRP Conjugate Diluent 20 µl   4684-096-06 10 mM Etoposide 100 µl   4822-96-08 TACS-SapphireTM 10 ml   *Components marked with an asterisk can be stored at -20°C for one year.   Storage: Components are stored at -80°C and 4°C.   References: 1. Lawen A. (2003) Apoptosis—an introduction. BioEssays 25:888-896. 2. Okada H, Mak TW. (2004) Pathways of apoptotic and non-apoptotic death in tumor cells. Nat. Rev. Cancer 4:592-603. 3. Yu S-W, Wang H, Poitras MF, Coombs C, Bowers WJ, Federoff HJ, Poirier GG, Dawson TM, Dawson VL. (2002) Mediation of poly(ADP-ribose) polymerase-1- dependent cell death by apoptosis-inducing factor. Science 297:259-263. Top   Ordering Information Catalog #: Price: 4684-096-K HT Colorimetric PARP/Apoptosis Assay, 96 Tests $495.00 Trevigen Products are now available for ordering through Download Protocol Related Products HT Chemiluminescent PARP/Apoptosis Assay HT Fluorescent Homogeneous PARP Inhibition Assay HT Chemiluminescent PARG Assay Kit HT Colorimetric PARG Assay Kit Universal Colorimetric PARP Assay Kit Universal Chemiluminescent PARP Assay Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM