Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage Universal Chemiluminescent PARP Assay Kit Poly ADP-ribosylation of nuclear proteins is a post-translational event that occurs in response to DNA damage. Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD-dependent addition of poly (ADP-ribose) to adjacent nuclear proteins. PARP plays an important role in DNA repair but can also lead to apoptosis by depleting the cellular NAD pool. Experimental models have shown that PARP inhibition prevents tissue damage in animal models of myocardial and neuronal ischemia, diabetes, septic shock, and vascular stroke. Recent data implicate a synergistic function of Ku80 and PARP-1 in minimizing chromosome aberrations and cancer development. Trevigen's Universal 96-well PARP Assay Kit measures the incorporation of biotinylated Poly (ADP-ribose) onto histone proteins in a 96-well plate format. This assay is ideal for the screening of PARP inhibitors and for measuring the activity of PARP in cell and tissue extracts. Features: Chemiluminescent, non-radioactive format High throughput 96 test size Sensitivity down to 0.0025 units of PARP per well Available with either a histone- coated plate or histone reagent   Applications: Identify inhibitors and activators of PARP activity Measure caspase inactivation of PARP Quantitate levels of DNA damage in cells caused by a variety of genotoxic agents Measuring activity of PARP in cell and tissue extracts   Cat# 4676-096-K HT Universal Chemiluminescent PARP Assay Kit w/ Histone Coated Strip Wells Components: Part # Component Size   4667-50-03 *200 mM 3-Aminobenzamide 60 µl   4671-096-02 *20X PARP Buffer 500 µl   4671-096-03 *10X PARP Cocktail** 300 µl   4671-096-04 10X Strep-Diluent 2.0 ml   4671-096-05 5X Coating Buffer 1.4ml   4671-096-06 *10X Activated DNA 300 µl   4675-096-01 PeroxyGlow™ A 6 ml   4675-096-02 PeroxyGlow™ B 6 ml   4678-096-P *Histone Coated Strip Wells (white) 96 wells   4800-30-06 Strep-HRP 30 µl   4668-50-01 *PARP-HSA 50 µl     *Components marked with an asterisk can be stored at -20°C for one year. **Contains biotinylated NAD and Activated DNA. Cat# 4675-096-K HT Universal Chemiluminescent PARP Assay Kit w/ Histone Reagent Components: Part # Component Size   4667-50-03 *200 mM 3-Aminobenzamide 60 µl   4667-50-07 *Histone Solution 700 µl   4671-096-02 *20X PARP Buffer 500 µl   4671-096-03 *10X PARP Cocktail** 300 µl   4671-096-04 10X Strep-Diluent 2.0 ml   4671-096-05 5X Coating Buffer 1.4 ml   4671-096-06 *10X Activated DNA 300 µl   4675-096-01 PeroxyGlow™ A 6 ml   4675-096-02 PeroxyGlow™ A 6 ml   4675-096-03 *White Strip Well Plate 96 wells   4800-30-06 Strep-HRP 30 µl   4668-50-01 *PARP-HSA 50 µl     *Components marked with an asterisk can be stored at -20°C for one year. **Contains biotinylated NAD and Activated DNA.   Storage: Components are stored at -80°C and 4°C.   References: Curtin NJ. 2005. PARP inhibitors for cancer therapy. Expert Rev Mol Med. 7:1-20. Kauppinen TM, Swanson RA. 2005. Poly(ADP-ribose) polymerase-1 promotes microglial activation, proliferation, and matrix metalloproteinase-9119-mediated neuron death. J Immunol. 174:2288-2296. Kim MY, Mauro S, Gevry N, Lis JT, Kraus WL. 2004. NAD-Dependent Modulation of Chromatin Structure and Transcription by Nucleosome Binding Properties of PARP-1. Cell 119:803–814. Tong WM, et al. 2002. Synergistic role of Ku80 and poly(ADP-ribose) polymerase in suppressing chromosomal aberrations and liver cancer formation. Cancer Res. 62:6990-6996. Top   Ordering Information Catalog #: Price: 4675-096-K HT Universal Chemiluminescent PARP Assay Kit w/ Histone Reagent, 96 Samples $415.00 4676-096-K HT Universal Chemiluminescent PARP Assay Kit w/ Histone Coated, 96 Samples $445.00 Trevigen Products are now available for ordering through Download Protocol Select Protocol 4675-096-K 4676-096-K Related Products Universal Colorimetric PARP Assay Kit HT Colorimetric PARP/Apoptosis Assay HT Chemiluminescent PARP/Apoptosis Assay HT Fluorescent Homogeneous PARP Inhibition Assay HT Colorimetric PARG Assay Kit HT Chemiluminescent PARG Assay Kit Anti-PAR Polymer Monoclonal Antibody Anti-PAR Polymer Polyclonal Antibody Anti-PARP Monoclonal Antibody Human PARP Enzyme (Standard) Human PARP Enzyme (HSA) High Specific Activity Human Poly(ADP-Ribose Polymerase Catalytic Domain (PARP-CAT) PARP Inhibitors FITC-NAD Biotinylated NAD Anti-PAR Polymer Monoclonal Affinity Purified Antibody TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM