| Source: |
Purified from E. coli containing a recombinant plasmid harboring the Paramecium bursaria Chlorella virus pdg gene. |
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| Unit Definition: |
One unit is the amount of enzyme required to completely relax 250 ng of a UV-irradiated supercoiled plasmid in 30 minutes at 37°C. |
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| Assay Conditions: |
1X RECTM Buffer 11 (25 mM NaPO4 (pH 6.8), 1 mM EDTA, 100 mM NaCl, 1 mM DTT, 0.1 mg/ml BSA), supercoiled plasmid (250 ng) irradiated with 100 J/m2 UV light, and serial dilutions of enzyme in a 20 µl reaction volume are incubated for 30 minutes at 37°C. |
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| Applications: |
FLARETM
Supercoiled DNA relaxation assay
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| Storage: |
Store at 4°C. The enzyme is supplied in 25 mM NaPO4 (pH 6.8), 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1 mg/ml BSA. |
