Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage CometAssayTM Control Cells Trevigen has developed a set of cell preparations containing different levels of DNA damage to be used with Trevigen’s CometAssay™ Kits.  In a typical comet assay, electrophoresis methods and differences in cell preparations create a significant source of variation in comet tail parameters.  Such variation sometimes makes it difficult to compare results between laboratories, and even within the same lab.  To overcome this problem, Trevigen scientists developed a set of stable control cell populations containing incremental levels of DNA damage for use when performing the CometAssay™.  These control cells, when electrophoresed in the CometAssay™, consistently produce comets with predetermined percents of DNA in the tails. The healthy control cell population (CC0) was treated with Etoposide under various conditions to increase the amount of damage in populations CC1, CC2, and CC3, respectively.  These cryopreserved control cells are designed to act as controls to standardize and compare alkaline electrophoresis methods between individual users and laboratories. Features: Asynchronous set of Jurkat cells with dose amounts of Etoposide   Applications: CometAssayTM controls for detection of DNA damage FLARE Assay controls for detection of DNA damage   Components: Part # Component Size   4256-010-CC0 Healthy Cells CC0 500 µl   4256-010-CC1 Treated Cells CC1 500 µl   4256-010-CC2 Treated Cells CC2 500 µl   4256-010-CC3 Treated Cells CC3 500 µl   Storage: Components are stored using a Liquid Nitrogen Storage System. Top   Ordering Information Catalog #: Price: 4256-010-CC CometAssay Control Cells, set $130.00 Trevigen Products are now available for ordering through Download Protocol Related Products CometAssay Kit Higher Throughput CometAssay CometAssay 96 CometAssay Silver Staining Kit DASH (Diffusion Apoptosis Slide Halo) Assay Kit cv-PDG FLARE Assay Kit and Module Endonuclease III FLARE Assay Kit and Module FLARE Assay Kits Fpg FLARE Assay Kit and Module hOGG1 FLARE Assay Kit and Module T4-PDG FLARE Assay Kit and Module UVDE FLARE Assay Kit and Module Download Presentation CometAssayTM Control Cells Presentation A B Results of a typical CometAssay™ experiment. DNA fragmentation associated with oxidative DNA damage was visualized using the CometAssay™ kit. (A) Damaged cell showing characteristic “puff” of DNA. (B) Untreated cell showing no DNA damage. Click the images below to download PDF files CometAssay™ Poster AACR University of Leicester   CometAssay™ Publication British Journal of Cancer   CometAssay™ Brochure   SOT 2008 Presentation   TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM