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CometAssay® Silver Staining Kit

Trevigen’s CometAssay® Silver Staining Kit is designed for the convenient silver staining of CometAssay® or single cell gel electrophoresis results. Recording of data generated by the CometAssay® and staining with fluorescent dyes requires epifluorescence microscopy and rapid analysis because of the generally short lifespan of the dye. Using the Silver Staining kit, permanent records are created and visualization using standard light microscopy is possible. The Silver Staining Kit was designed specifically for use with the CometSlides to minimize unwanted background and the amount of hazardous waste generated by silver nitrate.

Features:
  • Convenient format.
  • Optimized for CometSlide staining.
  • Reduced hazardous waste.
  •  
    Applications:
  • CometAssay®
  • DNA Damage detection
  •  
      Cat# 4254-200-K CometAssay® Silver Staining Kit
    Components: Part # Component Size
      4254-200-01 20X Staining Reagent #1 1.2 ml
      4254-200-02 20X Staining Reagent #2 1.2 ml
      4254-200-03 20X Staining Reagent #3 1.2 ml
      4254-200-04 2X Staining Reagent #4 1.2 g
      4254-200-05 10X Fixation Additive 2.2 ml
     
     
      Cat# 4251-050-K CometAssay® Silver
    Components: Part # Component Size
      4250-050-01 CometAssay® Lysis Solution 2 x 500 ml
      4250-050-02 CometAssay® LMAgarose 15 ml
      4250-050-03 CometSlide (2 well slide) 25 each
      4250-050-04 200 mM EDTA; pH 10 12.5 ml
      4254-200-01 20X Staining Reagent #1 1.2 ml
      4254-200-02 20X Staining Reagent #2 1.2 ml
      4254-200-03 20X Staining Reagent #3 1.2 ml
      4254-200-04 2X Staining Reagent #4 1.2 g
      4254-200-05 10X Fixation Additive 2.2 ml
     
     
    Storage: Store (cat# 4254-200-K) at room temperature.


    * When reconstituted and stored at 4°C, component is stable for 3 months.
    ** All Components in the CometAssay® Silver Staining Kit (4254-200-K) are also included in the CometAssay® Silver (4251-050-K).
    *** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.
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    Ordering Information
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    Catalog #: Price:

    4251-050-K
    CometAssay Silver Kit,
    50 Samples
    $315.00
    Add to Cart

    4254-200-K
    CometAssay Silver Staining Kit,
    200 Samples
    $160.00
    Add to Cart

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    A
    SYBR® Green I nucleic acid gel stain
    B
    TrevigenÂ’s CometAssay Silver Staining Kit
    Comets visualized using either ***SYBR® Green I nucleic acid gel stain (A) or TrevigenÂ’s CometAssay® Silver Staining Kit (B). Potassium Permanganate treated WEHI 7.1 cells were processed using the CometAssay® kit. Samples were fixed in cold ethanol, and air dried. Following drying, the slides were stained with ***SYBR® Green I nucleic acid gel stain. Subsequently, the slides were stained with the CometAssay® Silver Staining Kit as recommended in the instructions.
     

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