| Applications: |
Immunodetection by Western and dot-blotting systems. Empirical determination will be required for optimal results. For Western blotting, we recommend a transfer time of 30-40 minutes at 25 volts and ten microliters of undiluted material. Our recommended concentration for dot blot analysis is to dilute 1/1000 and use 100 microliters.
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| Storage: |
PAR is provided in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA and is stable for at least 1 year at -80°C. It may be aliquoted to avoid repeated freezethawing. |
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| Reference: |
1. Affar, E.B., et al. 1998. Immunodot blot method for the detection of poly(ADP-ribose) synthesized in vitro and in vivo. Anal Biochem 259:280-283.
2. Shah, G.M., et al. 1995. Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo. Anal Biochem 227:1- 13.
3. Menard, L. and G.G. Poirier. 1987. Rapid assay of poly(ADP-ribose) glycohydrolase. Biochem Cell Biol 65:668-673.
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