Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage Human PARP Enzyme (HSA) High Specific Activity Poly ADP-ribosylation by PARP of nuclear proteins is a post-translational event that occurs in response to DNA damage. PARP inhibition prevents tissue damage in animal models of myocardial and neuronal ischemia, diabetes, septic shock, and vascular stroke. High specific activity PARP is ideal for use as a positive control in Trevigen’s Universal PARP Assay Kits, for detailed mechanistic studies, in Western blot analysis of PARP in cell extracts, and for drug discovery applications. Unit Definition: One unit is the amount of enzyme required to incorporate 100 pmol of poly(ADP) from NAD into acid-insoluble form in 1 minute at 22°C.   Source: Purified from E. coli containing a recombinant plasmid encoding the full-length human PARP gene. PARP-HSA is greater than 95% pure by SDS-PAGE and Coomassie staining.   Applications: Identification of inhibitors and activators of PARP activity Quantitation of DNA damage Investigation of PARP inactivation during apoptosis   Storage: Freeze in working aliquots at -80 C to avoid repeated freeze-thawing. Top   Ordering Information Catalog #: Price: 4668-100-01 Recombinant Human PARP Enzyme (HSA), 1,000 Units $245.00 4668-500-01 Recombinant Human PARP Enzyme (HSA), 5,000 Units $850.00 4668-2K-01 Recombinant Human PARP Enzyme (HSA)*, 20,000 Units $2900.00 Trevigen Products are now available for ordering through Download Protocol Related Products Human PARP Enzyme (Standard) Anti-PAR Polymer Polyclonal Antibody Anti-PARP Monoclonal Antibody HT Colorimetric PARG Assay Kit Biotinylated NAD FITC-NAD Universal Colorimetric PARP Assay Kit Universal Chemiluminescent PARP Assay Kit PARP Inhibitors Anti-PAR Polymer Monoclonal Antibody TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM