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Molecular Biology

Klenow Enzyme

The large fragment of E. coli DNA polymerase I (Klenow) is a DNA polymerase with an associated 3’ to 5’ exonuclease activity and lacks any 5’ to 3’ exonuclease activity. This enzyme is used in a variety of experiments including DNA end labeling of 3’ recessed ends and DNA sequencing.

Concentration: 15 units/µl.
 
Unit Definition: 1 unit incorporates 10 nmole of total nucleotide into acid-insoluble form in 30 minutes at 37°C using activated DNA as a template/primer.
 
Applications:
  • DNA end labeling of 3' recessed ends
  • In situ labeling of apoptotic cells
  •  
    Source: Purified from a recombinant E. coli strain.
     
    Storage: Store at -20°C in a manual defrost freezer to avoid repeated freeze-thawing.
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