Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Oxidative Damage Oxidative Damage HT Glutathione Assay Kit Trevigen’s Glutathione assay kit utilizes a carefully optimized enzymatic recycling method, using Glutathione reductase, for the quantification of Glutathione. The sulfhydryl group of Glutathione reacts with DTNB (5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by Glutathione reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to this recycling reaction which is in turn directly proportional to the concentration of GSH in the sample. Measurement of the absorbance of TNB at 405 or 414 nm provides an accurate estimation of Glutathione in the sample. Features: Suitable for mammalian cells, tissue, blood, plasma and other bodily fluids Formatted for 96-well plates Contains sufficient reagents to assay 384 data points or to determine GSH in: 123 experimental samples, each performed in triplicate, plus one GSH standard curve 108 experimental samples, each performed in triplicate, plus 4 GSH standard curves 88 experimental samples, each performed in triplicate, plus 8 GSH standard curves Includes procedure for determining oxidized and reduced glutathione   Applications: Calculating total, reduced, and oxidized glutathione concentrations.   Components: Part # Component Size   7511-100-01 Glutathione Reductase 80 µl   7511-100-02 25X Assay Buffer 12 ml   7511-100-04 Reaction Mix 8 bottles   7511-100-05 96-Well Plates 8 plates   7511-100-06 4 µM GSSG 2.5 ml   Storage: Store components at 4°C. Top   Ordering Information Catalog #: Price: 7511-100-K HT Glutathione Assay Kit, 384 tests $315.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products HT Superoxide Dismutase Assay Kit HT Glutathione Reductase Assay Kit Superoxide Dismutase Assay Kit Glutathione Reductase Assay Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP