Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » Oxidative Damage Oxidative Damage Glutathione Reductase Assay Kit Trevigen is pleased to announce the release of a new, higher volume assay kit designed to measure cellular levels of Glutathione Reductase, an enzyme which catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). GSH is an important intracellular antioxidant that reacts with oxygen free radicals and organic peroxides and acts as a substrate for other detoxification enzymes such as glutathione peroxidase and glutathione S-transferase. The activity of glutathione reductase is an important measure of the antioxidant status of the cell. Glutathione reductase requires NADPH for its activity, resulting in the reduction of GSSG to GSH and the corresponding oxidation of NADPH to NADP. Trevigen’s Glutathione Reductase Assay takes less than 6 minutes to perform and is based on the measurement of the loss in absorbance at 340 nm due to the conversion of NADPH to NADP+. The kit contains everything that you need to perform the assay on 100 samples and its corresponding blanks, each in duplicate, as well as 10 standard curves with each point performed in duplicate. In total, each kit can generate data on over 500 data points. The NADPH is provided lyophilized in 10 vials, each containing sufficient NADPH for generating one standard curve and for determining glutathione reductase levels in 10 experimental samples. Features: Suitable for cell lysates, erythrocyte lysates, and tissue homogenate Each sample takes only 6 minutes Sufficient reagents for 100 assays and 10 standard curves   Applications: Calculating glutathione reductase activity.   Components: Part # Component Size   7510-100-01 Glutathione Reductase 500 µl   7510-100-02 25X Glutathione Reductase Reaction Buffer 20 ml   7510-100-03 NADPH 10 x 9.2mg   7510-100-04 GSSG Solution 6 ml   7510-100-05 25X Sample Dilution Buffer 20 ml   7510-100-06 25X Tissue Homogenization Buffer 40 ml   Storage: Store components at 4°C. NADPH should be stored at -20°C. Top   Ordering Information Catalog #: Price: 7510-100-K Glutathione Reductase Assay, 100 reactions $435.00 Trevigen Products are now available for ordering through Download Protocol Related Products HT Superoxide Dismutase Assay Kit HT Glutathione Reductase Assay Kit Superoxide Dismutase Assay Kit HT Glutathione Assay Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM