Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » Oxidative Damage Oxidative Damage HT Glutathione Reductase Assay Kit This high throughput glutathione reductase assay kit is designed to measure cellular levels of Glutathione Reductase, an enzyme which catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). GSH is an important intracellular antioxidant that reacts with oxygen free radicals and organic peroxides and acts as a substrate for other detoxification enzymes such as glutathione peroxidase and glutathione S-transferase. The activity of glutathione reductase is an important measure of the antioxidant status of the cell. Glutathione reductase requires NADPH for its activity, resulting in the reduction of GSSG to GSH and the corresponding oxidation of NADPH to NADP. Trevigen's Glutathione Reductase Assay Kit is a spectrophotometric assay in which the oxidation of NADPH to NADP+ is monitored by the decrease in absorbance at 340 nm. This rate of decrease in absorbance at 340 nm is directly proportional to the glutathione reductase activity in the sample because the enzyme is present at rate limiting concentrations [2-4] The kit provides the user with all the reagents and plates to easily and rapidly assay for glutathione reductase in cell and tissue extracts. Features: Suitable for cell lysates, erythrocyte lysates, and tissue homogenate Sufficient reagents for 500 assays 96 well format for high throughput screening   Applications: Calculating glutathione reductase activity.   Components: Part # Component Size   7510-100-03 NADPH 10 x 9.2mg   7512-100-06 96-well plates 5 plates   7513-500-01 Glutathione Reducatase 1 ml   7513-500-02 10X GR Buffer 20 ml   7513-500-03 GSSG 3 ml   Storage: Store components at -20°C, 4°C, and room temperature. Top   Ordering Information Catalog #: Price: 7513-500-K HT Glutathione Reductase Assay Kit, 480 tests $500.00 Trevigen Products are now available for ordering through Download Protocol Related Products HT Superoxide Dismutase Assay Kit Superoxide Dismutase Assay Kit Glutathione Reductase Assay Kit HT Glutathione Assay Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM