Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Oxidative Damage Oxidative Damage Superoxide Dismutase Assay Kit Superoxide Dismutase (SOD) catalyzes the dismutation of the superoxide radical (O2-) into hydrogen peroxide (H202) and elemental oxygen (O2), and as such, provides an important defense against the toxicity of superoxide radical. Overexpression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation (1). SOD also inhibits adriamycin-induced apoptosis in murine peritoneal macrophages (2). Superoxide ions, generated from the conversion of xanthine to uric acid and hydrogen peroxide by xanthine oxidase, converts NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SOD reduces the superoxide ion concentration and thereby lowers the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in your experimental sample. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. The kit contains the proper lysis buffer and the reagents needed for 100 experimental tests, 50 positive controls, and 50 negative controls. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals. Each assay requires only about five minutes, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample. Features: Suitable for mammalian cells Each sample takes only 5 minutes Contains SOD for 50 positive controls Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD   Applications: Calculating SOD activity by measuring the reduction of NBT-diformazan in cell extracts.   Components: Part # Component Size   7500-100-01 SOD Standard, 50 units/µL 200 µl   7500-100-02 25X SOD Reaction Buffer 12 ml   7500-100-03 Xanthine Solution 1.5 ml   7500-100-04 NBT Solution 6.0 ml   7500-100-05 XOD Solution 2.0 ml   7500-100-06 20X Cell Lysis Solution 12.0 ml   Storage: Store components at 4°C.   References: 1. Zhao, Y., K.K. Kiningham, S.M. Lin, and D.K. St. Clair. 2001. Overexpression of MnSOD protects murine fibrosarcoma cells from apoptosis and promotes a differentiation program upon treatment with 5- azacytidine. Antioxid. Redox Signal. 3:375-386. 2. Dominguez-Rodriguez, J.R., et.al. 2001. In vivo inhibition by antioxidants of adriamycin-induced apoptosis in murine peritoneal macrophages. Anticancer Res. 21:1869-1872. Top   Ordering Information Catalog #: Price: 7500-100-K SOD Assay Kit, 100 reactions $480.00 Trevigen Products are now available for ordering through - AND - Download Protocol Related Products HT Superoxide Dismutase Assay Kit HT Glutathione Reductase Assay Kit Glutathione Reductase Assay Kit HT Glutathione Assay Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP